The azopyrum probe is a control of the quality of the presterilization cleaning of a medical instrument for the presence of microscopic elements, carried out with the help of a special solution, which is applied to the surface with the aid of cotton wool or a pipette. If the solution is acquired a certain shade, it means that the medical product was poorly cleaned of blood, particles of skin and rust. Next, you will learn how to make an azo-pyramidal sample and what it is.
What is the azoepiam test
The medical instrument is thoroughly cleaned and sterilized before carrying out the operation. This process is not as simple as it seems at first glance. To start scalpels, syringes and other objects that have direct contact with the person being operated, soak for several hours in a special solution. This is how the pre-sterilization procedure takes place, followed by strict control.
After that, the instrument is subjected to the most sterilization, in which all microbes and viruses are destroyed. However, this procedure does not remove the remnants of organic matter from the instrument, for example, blood particles or pieces of tissue that have not been washed with a pre-sterilization solution. Remnants of organic substances or rust can cause unnecessary reaction when entering the body of the person to be operated. And it can do harm to tools. Azipira is used to test the quality of precautionary testing.
Indications for
The azopyramic test of this substance evaluates the quality of cleaning of medical devices and instruments. Precision does not give a 100% guarantee of cleaning the scalpels and clamps. Azo-pyramid solution does not clean the surface, it only shows if there are any remaining blood, rust and other microscopic elements on the instrument that are not visible to the human eye. During the control by means of a sample it is possible to detect the presence of residues of such substances:
- blood;
- human skin;
- muscle tissue;
- rust;
- soap residue;
- chloramine;
- bleaching powder;
- bleach;
- acid;
- oxidants of vegetable origin.
Components of azopyramide sample
Azopyram is a chemical reagent for detecting the presence of contaminants on a medical instrument. It is available in plastic bottles, which contain such substances:
- Amidopyrin. This substance is also called pyramidone. Amidopyrine is a clear liquid without color with a slightly noticeable odor. In dry form it has the appearance of a white powder. The main fields of application of amidopyrine are the production of paints, explosives, photos and medicine. At the beginning of the 20th century, this substance was used to relieve temperature and headache.
- Aniline hydrochloric acid. This substance is poisonous, it should be operated with care. Aniline is volatile, it can not be kept near heating objects in an open condition.
What is azipyram
A sample of azopiram is carried out using a special reagent that includes azopyram itself and 3% hydrogen peroxide diluted in equal proportions. Azopyram is made from the above components: amidopyrine and aniline. In medical institutions, the components for research are purchased by parties. To create a reagent is sometimes used ethyl alcohol.
To properly dilute the substance, you must strictly follow the instructions. Azopyram is not the only component that is used in medicine to analyze the surface by changing the color or separating the components. For example, a phenolphthalein solution familiar to everyone from school lessons is an indicator of an acidic or alkaline medium.
Preparation of
Solution Azopyram is available in different forms: A set of Azopyram and dry powder concentrate( SC Azopiram).In the first case, it is necessary to pour the contents of the bubble with aniline into a solution of amidopyrine in order to obtain a reagent. To prepare one liter of sample solution from a dry powder, one hundred grams of amidopyrine and one and a half grams of aniline are taken, which are diluted with ethyl alcohol to volume and mixed. The resulting starting reagent can not be used in pure form, it is diluted with hydrogen peroxide.
How to test the suitability of a solution
Before use, the working solution is tested for suitability at room temperature by applying to a test sample. The test is not lengthy, it is done within one minute. To do this, it is required to collect azopirs in a pipette and drop onto a stain from the blood. If after one minute the solution has acquired a purple color, then the substance is prepared correctly, it is suitable for testing a medical instrument.
Technique for the work of
The azopyramic test is performed on the medical device according to the instructions. Particular attention is paid to safety techniques, since solution components, for example aniline, are hazardous to health. This should be considered before working with the reagent. Below is a step-by-step algorithm for analyzing the cleaning of a medical instrument.
Algorithm
To carry out the sample, the following equipment will be required:
- kit of Azopyrim or SC Azopyram with ethyl alcohol;
- gloves and mask for medical personnel;
- set of pipettes;
- cotton swabs;
- container;
- itself is a medical instrument that has undergone a procedure of pre-sterilization.
Once all the items necessary for analyzing the quality of cleaning the medical instrument are ready, you can proceed with the procedure. The algorithm for carrying out the azopyramic test is as follows:
- At the first stage, the workplace is arranged, gloves and a mask are put on.
- Next, a solution of azopiram is prepared. There are two methods of dilution of the substance. If the kit is used, then you only need to connect the two components together: the amidopyrine solution and the hydrochloric acid aniline. To prepare the reagent from the dry amidopyrine, it is necessary to measure the required amount of substances and dissolve them in ethyl alcohol.
- Preparation of a working solution, which consists of a mixture of azopyram and 3% hydrogen peroxide.
- Before the test, a preliminary test is made by the test for the suitability of the solution, which is carried out on a drop of blood, which is dripped onto the surface of the napkin. If the solution stains, it means that it is possible to analyze the purity of the instrument.
- To determine the contamination, a working solution of azopiram is applied to the medical instrument with a pipette. Sometimes the surface should be wiped with a clean cloth, which must first be moistened in a liquid. If the treatment is carried out with reusable syringes, the azopyramic solution is taken up by means of a piston.
- Wait a minute: change the color of the solution or not? To make a conclusion.
- After completion of the test, you should thoroughly wash your hands with warm water and soap.
Evaluation of results
To determine the quality of tool cleaning is easy. If microparticles of blood or other substances remain on the surface, the color of the solution will change at this point. It can be painted in purple, lilac, pink, bard or brown. If such a reaction is found, it is said that the test is positive and the tool needs to be re-pre-inspected. If the drug has not changed color, then there is no staining, the test is considered negative. All data on the sample is recorded in a special journal with the content of the research.
Basic Rules for Conducting the
Test During the procedure, you must strictly follow the safety procedures, especially for aniline. Staff without protective equipment is not allowed to work. Aniline hydrochloride is very dangerous, it can lead to toxic hepatitis. Penetrating into the body, this component of the solution causes nausea, vomiting, dizziness, weakness, increased heart rate. Poisoning can also be said on the skin: the fingers and tips acquire a bluish tinge.